England captain Harry Kane is set to have a scan on his right ankle before Friday's World Cup game with the United States.
He was eventually replaced by Callum Wilson and was later seen with light strapping on the ankle.
Kane is set to have the scan on Wednesday to assess the extent of the problem.
The Tottenham striker has scored 51 goals in 76 appearances for the Three Lions.
England boss Gareth Southgate will hope Kane's scan results are positive as he prepares his team for the second game of the group stage.