The Tottenham Hotspur Supporters' Trust has urged the club's board to resign after their involvement in an attempt to form a European Super League.
However, all six withdrew their involvement on Tuesday following resistance to the plans.
In a vote on Friday evening, 90% of the Trust's membership said the board had to resign immediately.
The Trust said in a statement: "The consequences of their decision to attempt to launch this breakaway league could now lead to substantial penalties against Spurs - points deductions, suspension from competition, financial penalties, other sanctions.
"They signed up to this plan knowing they risked all that, and knowing they risked their players being banned from international competition.