Fans at the Tottenham Hotspur Stadium were heard shouting during the minute's silence ahead of Tottenham's Premier League clash with Leicester on Saturday afternoon.
The minute's silence - which was followed by a rendition of the national anthem - was punctuated by audible shouts from supports, although it is unclear whether they were fans of the home or away side.
Tribute was being paid to Queen Elizabeth II with this weekend's round of fixtures the first since her passing on Thursday September 8.
And the actions of a select few in attendance have sparked outrage on Twitter, with those to break the show of respect harshly criticised on the social media platform.