Wimbledon has banned Russian and Belarusian players because it is not prepared "to be used to benefit the propaganda machine of the Russian regime", says chairman Ian Hewitt.
Wimbledon runs from 27 June to 10 July.
"If conditions significantly change, we would happily review. At this point, we're very clear about our intent," chief executive Sally Bolton added.
Meanwhile, the organisers of the grass-court Grand Slam also announced that players who are not vaccinated against Covid-19 will be able to compete at the All England Club.
Serbia's Novak Djokovic, the men's world number one and defending Wimbledon champion, was not allowed to play at the Australian Open in January following a row which centred around him being unvaccinated.