Top seed Angelique Kerber will lose her world number one ranking after being beaten by Spain's Garbine Muguruza in the fourth round at Wimbledon.
Muguruza, the 14th seed and a runner-up at SW19 in 2015, won an enthralling match 4-6 6-4 6-4 in two hours and 20 minutes on court two.
The German, a finalist in 2016, had needed to reach Saturday's final to have a chance of retaining her ranking.
Muguruza, 23, will play Svetlana Kuznetsova of Russia in the last eight.
Big-hitting Muguruza was encouraged to go on the attack by her coach Conchita Martinez, the 1994 champion who is still the only Spanish woman to win Wimbledon.