Second seed Garbine Muguruza is out of Wimbledon after losing the second round in straight sets to Slovakia's world number 124 Jana Cepelova on Thursday.
The 22-year-old French Open winner, who lost last year's final to Serena Williams, lost 6-3 6-2 on Court One.
It is a second big scalp for Cepelova in as many years at Wimbledon, after knocking out world number three Simona Halep in the first round last year.
The 23-year-old will face Czech 29th seed Lucie Safarova in the third round.