Jo-Wilfried Tsonga became the latest seed to fall at the Aegon Championships with a straight-set defeat by Luxembourg's Gilles Muller.
The Frenchman, seeded fifth, lost 6-4 6-4 in the second round at Queen's.
Tsonga's exit follows defeats for Andy Murray, Stan Wawrinka and Milos Raonic - the top three seeds - on Tuesday.
Croatia's fourth seed Marin Cilic and Bulgaria's sixth seed Grigor Dimitrov are the leading players left in the draw at the London tournament.
Dimitrov takes on France's Julien Benneteau later on Wednesday, before Czech seventh seed Tomas Berdych plays Canadian teenager Denis Shapovalov.