British number one Johanna Konta missed out on a place in the main draw of the WTA event in Linz with a three-set defeat by Czech Klara Koukalova.
Konta, at a career-high ranking of 47, led 4-2 in the final set but lost 6-2 2-6 6-4 to the world number 105.
It was only Konta's third defeat in 26 matches since Wimbledon, and her first to a lower-ranked player since May.
The 24-year-old will play doubles with fellow Briton Naomi Broady in Linz, before heading to Luxembourg next week.
Konta had followed up her remarkable run to the quarter-finals in Wuhan, China, where she beat world number two Simona Halep before losing narrowly to Venus Williams, with two qualifying wins in Linz.