Britain's Sonay Kartal missed the chance to reach a home quarter-final when she suffered a tough defeat by American Amanda Anisimova at Queen's.
World number 50 Kartal was overpowered by the eighth seed in just one hour and five minutes, losing 6-1 6-3.
Kartal had secured the biggest win of her career on Monday against world number 16 Daria Kasatkina.
But a third win over a top-20 player in her career - and a second in the west London tournament - proved a bridge too far.
Anisimova will not have won over much of the British crowd so far at Queen's with victory over Jodie Burrage in the first round and now Kartal.