There will be no British women in the French Open main draw after Katie Boulter, Harriet Dart and Fran Jones exited in the second qualifying round.
British number three Boulter was beaten 6-2 2-6 7-6 (10-3) by American 19-year-old Ashlyn Krueger at Roland Garros.
Dart fell to a 1-6 6-1 6-2 loss to France's Elsa Jacquemot, while Jones had to retire against Ylena In-Albon
It will be the first time no British woman has featured in the main draw at a Grand Slam since the 2009 US Open.
World number 316 Jones, who beat Coco Vandeweghe on Monday, was left in tears after the 22-year-old was forced to retire because of a shoulder injury when trailing 4-1 in the first set.