Harriet Dart and Fran Jones were among the British players who saw their French Open hopes ended in the second qualifying round on Wednesday.
British number four Dart won the first set before falling to a 1-6 6-1 6-2 loss to France's Elsa Jacquemot.
World number 316 Jones was left in tears after she was forced to retire from her match against Ylena In-Albon.
The 22-year-old, who stunned former grand slam semi-finalist Coco Vandeweghe on Monday, had trailed 4-1.
Jones, unable to continue because of a shoulder injury at Roland Garros, has a congenital condition which means she is missing one finger on each hand and three toes.