Four-time Grand Slam champion Naomi Osaka was beaten by Amanda Anisimova in a high quality first-round encounter at the French Open.
American Anisimova, who ended Osaka's Australian Open title defence in January, won 7-5 6-4.
Osaka had struggled with an Achilles injury in the build-up to the Grand Slam but was let down most by her serve, including eight double faults.
Anisimova, a semi-finalist in 2019, will face Croatia's Donna Vekic next.
Osaka said she had initially worried about the reception she would receive on her return to Paris after withdrawing from last year's event because of mental health struggles.