Human line judges will be replaced by an electronic calling system on a full-time basis on the ATP Tour from 2025.
The ATP, which is the men's governing body, said the move will "optimise accuracy and consistency" in events.
Some tournaments have already scrapped human line judges, including the majors at the Australian Open and US Open.
"This is a landmark moment for our sport, and not one we've reached without careful consideration," said ATP chairman Andrea Gaudenzi.
"Tradition is core to tennis and line judges have played an important part in the game over the years.