Papa John's Trophy holders Sunderland were knocked out of the competition after their 1-0 defeat at home to out-of form Oldham Athletic.
Harry Vaughan's second-half goal, which came following Cieran Dunne's defensive mishap, sent Oldham to the last 16.
The Latics are without a manager and 23rd in League Two, having lost seven of 10 games coming into the tie.
Elsewhere, Arsenal Under-21s came from 2-0 down to hold Ipswich to a 2-2 draw before winning 4-3 on penalties.
Kayden Jackson scored twice at Portman Road to send the Tractor Boys into a half-time lead, but an own goal from Kane Vincent-Young and an equaliser from Folarin Balogun three minutes later took it to spot-kicks.