It was another difficult night for Premier League academy sides in the EFL Trophy.
Southampton Under-21s fell to a 4-1 defeat at League One strugglers Charlton, who were playing in front of a record low home attendance of 689.
The Saints' goal was scored by 32-year-old player-coach Olly Lancashire, but it could not stop them slipping to a second successive defeat in the competition.
Liverpool's under-21 side, including defender Nat Phillips, 24, were beaten 4-1 at Bolton Wanderers while the youth teams of Leicester, Newcastle and Wolves lost at Accrington, Harrogate and Shrewsbury respectively.
The Foxes slumped to a 5-0 loss at Stanley and have conceded nine goals in their two defeats.