Aston Villa's Under-21 side will take on Charlton Athletic in the last 32 of the EFL Trophy.
The Premier League academy side are so far unbeaten in the competition and topped their group to secure a home tie against the League One Addicks.
Chelsea's Under-21s face a trip to League Two leaders Forest Green Rovers, while Arsenal Under-21s have also been drawn away - at Ipswich Town.
Last years' beaten finalists Tranmere Rovers also have a home tie against Harrogate Town, with 2019 winners Portsmouth drawn away at League Two high-flyers Exeter City.
The teams remain divided into northern and southern draws, with the ties to be played during the week of 29 November.