Southampton boss Ralph Hasenhuttl said it was a "risk" to play Danny Ings after he suffered a hamstring injury in the 1-0 defeat by Manchester City.
Ings, who has scored six goals this season, was playing his fourth game in 13 days for the Saints.
"We had one day less to recover. It was a risk to play him. Hopefully he's not so bad," said boss Hasenhuttl.
The Austrian told BBC Match of the Day that the club will "have a look" at the extent of Ings' injury.
Southampton have had an impressive season and their form has seen them competing for a top-four place.