James Ward-Prowse and Nathan Redmond have returned to the England Under 21s for a training camp ahead of Euro 2017.
The Southampton pair earned their first senior caps against Germany in March and June's tournament in Poland will be their last appearances for the Young Lions before they become too old.
Manchester City's Patrick Roberts, who has spent this season on loan at Celtic, has been handed a first call-up while Sunderland's Jordan Pickford is also in.
But Manchester United striker Marcus Rashford has been included in the senior squad and is now not expected to travel to Poland with the Young Lions.