The 38-year-old, who has spent his entire professional career with the Giallorossi, reached the historic milestone with his 244th strike in Serie A.
Roma captain Francesco Totti scored his 300th goal for the club in Sunday's Serie A clash with Sassuolo at the Stadio Olimpico.
The former Italy international, who turns 39 next week, reached the milestone nine minutes before halftime when he whipped home a pass from Miralem Pjanic.
Sassuolo, though, was furious that the goal was allowed to stand, as Totti had clearly been standing in an offside position when he was picked out by his Bosnian teammate on the edge of the area.