The Argentine and the club have parted ways after a third consecutive defeat.
Pablo Marini is no longer the manager of Puebla, the club announced Monday afternoon.
The news came two days after a 4-1 loss to Leon, the third consecutive defeat for La Franja and the seventh straight week without a victory.
Marini came to the club in 2015 and was able to guide the team to victory over Morelia in the one-off SuperCopa MX in 2015. But Puebla was unable to see off Racing in the first round of the Copa Libertadores after earning their spot with the SuperCopa victory.