Spain coach Vicente del Bosque has left Saul Niguez and Isco out of his final 23-man squad for Euro 2016.
The reigning champions named a 19-man group earlier on Tuesday, leaving four places available for six contenders from Atletico Madrid and Real Madrid following the Champions League final.
Sergio Ramos, Koke, Lucas Vazquez and Juanfran have made the cut, with midfield duo Isco and Saul missing out.
The omission latter comes as a significant surprise, with the 21-year-old midfielder having been a key part of Atletico's journey to the Champions League final and a third-placed La Liga finish.