Marcelo has dropped out of the Brazil squad for friendlies against Saudi Arabia and Argentina, the Brazilian FA (CBF) has confirmed.
The Real Madrid left-back has missed his team's last two matches, a 0-0 derby draw with Atletico Madrid in La Liga and Tuesday's 1-0 Champions League loss away to CSKA Moscow.
And Marcelo's calf injury will prevent the 30-year-old from linking up with Tite's squad during the international break, the CBF said on Wednesday.
The World Cup quarter-finalists are yet to confirm whether they will call up a fresh face to replace Marcelo, who has made 58 appearances to date for his national team.