The Seattle Sounders lost for the first time in the CONCACAF Champions League this season, while San Francisco upstaged Queretaro.
The Seattle Sounders' unbeaten CONCACAF Champions League record came to an end against Olimpia, as the MLS side fell 1-0 in Honduras on Wednesday.
The Sounders registered their first win in Group F courtesy of a late 3-2 win over Olimpia last week.
Stars Obafemi Martins and Clint Dempsey were absent from that match and Sigi Schmid named the same starting XI for the reverse fixture at Estadio Tiburcio Carias Andino, but Seattle was unable to repeat the feat as Alberto Elis sank the MLS visitors with his strike on the hour mark.