Tottenham have "unequivocally rejected" an informal expression of interest from a consortium led by American tech entrepreneur Brooklyn Earick.
It is the third expression of interest rejected by the club's board since the sudden departure of executive chairman Daniel Levy earlier this month.
In a statement, ENIC Sports & Developments Holdings Ltd - which has an almost 87% stake in Tottenham - said it wanted to "reconfirm that Tottenham Hotspur is not for sale and ENIC is not looking to sell its stake in the club".
Earick has fuelled speculation by posting an image of the dressing room at the Tottenham Hotspur Stadium on social media, including a timer counting down the 27 days to 24 October.