Major League Soccer released its schedule for the 2021 season on Wednesday with each of the league's 27 clubs set to play 34 games, 17 home and 17 away, over the course of the regular season. Here are all the details on how the two conferences will be aligned and how the schedule has been constructed for the coming season.
| Atlanta United | Austin FC |
| Chicago Fire FC | Colorado Rapids |
| FC Cincinnati | FC Dallas |
| Columbus Crew SC | Houston Dynamo FC |
| D. |