Diogo Dalot and Bruno Fernandes have a UEFA Nations League semi-final to prepare for, as Portugal face Germany on Thursday night (20:00 BST kick-off).
The Allianz Arena encounter presents an opportunity for Roberto Martinez's men to push for more silverware after winning the inaugural event in 2018/19.
Nuno Tavares has been cut from the squad, leaving 26 players for Martinez to choose from, with Dalot making his comeback from injury in the Europa League final against Tottenham Hotspur.
The other last-four tie is between Spain and France, and takes place on Thursday night.