Missy Bo Kearns and Ceri Holland were on the scoresheet at Prenton Park to clinch an important set of three points for Matt Beard's side.
Kearns opened the scoring in the 62nd minute of the contest, as Holland quickly followed up to mark her 50th appearance for the Reds with a well-taken goal.
Liverpool would have to withstand a flurry of early pressure. Only the crossbar denied Reading grabbing a seventh-minute lead, Emma Harries' flicked header from Emma Mukandi delivery striking the woodwork and bouncing over.
At the other end, Holland's head back across goal nearly saw Katie Stengel pounce on a loose ball inside the area, though the danger was cleared away in the nick of time.