Defender Ben Chilwell has withdrawn from the England Under-21 squad to face Scotland and Andorra.
The Leicester left-back has returned to his club for treatment on an unspecified injury and while it is not serious he will not feature in the Young Lions' latest Euro 2019 qualifiers.
Arsenal's Ainsley Maitland-Niles and Everton defender Jonjoe Kenny missed training at St George's Park on Wednesday as they have treatment for minor knocks but remain in contention to face Scotland on Friday.
Aidy Boothroyd's side welcome their rivals to Middlesbrough's Riverside Stadium with England joint top of Group 4 and Scotland a point behind in third.