Paraguay will face Costa Rica, Colombia and the United States next month without 31-year-old forward Lucas Barrios.
Experienced Paraguay forward Lucas Barrios did not make the cut for the Copa America Centenario, overlooked by coach Ramon Diaz on Monday.
Barrios, who scored two goals at last year's Copa America in Chile, was included in Paraguay's provisional squad, having featured in World Cup qualifiers against Brazil and Ecuador in March. However, there was no room for the 31-year-old Palmeiras attacker in the final 23-man squad ahead of the century edition of the tournament, which will kick-off in the United States on June 3.