Diego Costa's eight-match suspension was upheld after the Royal Spanish Football Federation (RFEF) confirmed Atletico Madrid's appeal had been dismissed.
The Spain international was sent off during the first half of Atletico’s 2-0 defeat to Barcelona last Saturday following an outburst aimed at referee Jesus Gil Manzano
The official wrote in his post-match report that Costa had insulted his mother and, after being shown a red card, grabbed his arm to try to stop him from booking team-mates Diego Godin and Jose Gimenez.
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It was confirmed on Thursday that Costa had been suspended for eight games and would consequently miss the rest of the season, though Atletico were given 10 working days to lodge an appeal.