Fans will be urged to travel to the stadium via bicycle or public transport and eat only vegan food when Spurs host Chelsea in the Premier League on 19 September.
It is part of a plan to make the game the world's first net zero carbon fixture at elite level.
The clubs are working with broadcaster Sky to cut emissions around the match.
Fans will also be urged to take positive steps to reduce their own carbon footprint.
Both sets of players will arrive at the stadium on coaches powered by biofuel, and will be drinking water from more sustainable cartons rather than plastic bottles.