The Clarets tasted defeat in their first game at Premier League 2 level as Wolves came from behind at the Aggborough Stadium to take the points from an entertaining curtain-raiser.
Burnley had earlier mounted a fightback of their own to lead through goals from Lewis Richardson and Max Thompson.
But Austin Samuel’s double either side of the break made it a losing return to action for Steve Stone’s side in their first game since March.
The Clarets have subsequently gained Category One status for their academy set-up and moved up to the second tier of PL2.