Bolton boss Phil Parkinson will check on the fitness of midfielder Darren Pratley before finalising his side to face Aston Villa at the Macron Stadium.
Pratley suffered a knock in training but will hope to be passed fit to face Steve Bruce's promotion-chasing side.
Chinedu Obasi and Connor Hall both scored for the Under-23s in their 3-1 victory over Barnsley on Monday.
But Parkinson told his pre-match press conference on Thursday that "they have no right to jump the queue" for a place in the first team.
Neil Taylor will miss out for the visitors after failing to recover from a groin injury suffered in the surprise 3-1 defeat to QPR on Tuesday night.
Bolton Wanderers
