Six teams from the American Conference earned victories on the opening day of the 2025 season.
Memphis struck for six goals in a 6-0 win over Southern Miss, while Charlotte (2-0 over UNCW), South Florida (1-0 over Furman) and Temple (3-0 over Iona) also secured clean sheets. In addition Rice defeated Houston Christian, 2-1, and Tulsa took down Central Arkansas, 2-1, on the road.
| Away | Home | Location |
|---|---|---|
| Liberty 3 | East Carolina 1 | Greenville, N.C. |
| Charlotte 2 | UNCW 0 | Wilmington, N. |