Barcelona have confirmed Thomas Vermaelen sustained a hamstring injury in the 5-0 win over Real Betis on Sunday.
The centre-back pulled up in discomfort in the 38th minute at the Benito Villamarin after chasing down Sergio Leon near the halfway line.
Vermaelen was replaced by Samuel Umtiti, who came on for his first La Liga appearance since December 2 after recovering from a hamstring problem of his own.
Barca later tweeted to say Vermaelen will undergo further tests on Monday to determine the severity of the problem.
"Thomas Vermaelen suffered a hamstring injury in his left leg.