The 22-year-old's remarkable form for Lyon this season has attracted the interest of Europe's biggest clubs, with Arsenal and Inter Milan keeping tabs.
Juventus, however, were considered favourites to land the France Under-21 international but have now been put off by his reported valuation.
Calciomercato have revealed that Tolisso's price has risen from £34m to £52m putting an end to the chances of joining the Italian champions.
Arsenal still have concerns over the long-term futures of Alexis Sanchez and Mesut Ozil and have been keeping a close eye on Tolisso.